FAM DBCO, 6-isomer
Catalog Number | R09-0008 |
Category | Fluorescein FAM |
Molecular Formula | C42H32N2O7 |
Molecular Weight | 676.71 |
Catalog Number | Size | Price | Quantity |
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R09-0008 | -- | $-- |
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Product Introduction
Fluorescein (FAM) is one of the oldest yet well known fluorescent dyes. This derivative of FAM contains cyclooctyne group (dibenzocyclooctyne, abbreviated as DBCO or ADIBO) for the conjugation with azides via copper-free, strain-promoted azide alkyne cycloaddition (spAAc).FAM DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. Azides react with DBCO spontaneously upon simple mixing. The reaction does not require any catalysts, and it is very efficient.
Chemical Information |
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Purity | NMR 1H, HPLC-MS (95%) |
IUPAC Name | N-[6-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-6-oxohexyl]-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxamide |
Canonical SMILES | C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCCCCNC(=O)C4=CC5=C(C=C4)C(=O)OC56C7=C(C=C(C=C7)O)OC8=C6C=CC(=C8)O |
InChI | InChI=1S/C42H32N2O7/c45-30-16-19-33-37(23-30)50-38-24-31(46)17-20-34(38)42(33)35-22-28(15-18-32(35)41(49)51-42)40(48)43-21-7-1-2-12-39(47)44-25-29-10-4-3-8-26(29)13-14-27-9-5-6-11-36(27)44/h3-6,8-11,15-20,22-24,45-46H,1-2,7,12,21,25H2,(H,43,48) |
InChI Key | QAATVTNRYVCIIR-UHFFFAOYSA-N |
Solubility | good in DMF, DMSO |
Appearance | orange solid |
- Product Specification
ε, L⋅mol-1⋅cm-1 | 75000 |
Fluorescence Quantum Yield | 0.9 |
Excitation | 494 |
Emission | 520 |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Computed Properties | |
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XLogP3 | 6.4 |
Hydrogen Bond Donor Count | 3 |
Hydrogen Bond Acceptor Count | 7 |
Rotatable Bond Count | 7 |
Exact Mass | 676.22095136 g/mol |
Monoisotopic Mass | 676.22095136 g/mol |
Topological Polar Surface Area | 125Ų |
Heavy Atom Count | 51 |
Formal Charge | 0 |
Complexity | 1340 |
Isotope Atom Count | 0 |
Defined Atom Stereocenter Count | 0 |
Undefined Atom Stereocenter Count | 0 |
Defined Bond Stereocenter Count | 0 |
Undefined Bond Stereocenter Count | 0 |
Covalently-Bonded Unit Count | 1 |
Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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