Atto 590 nhs ester

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Atto 590 nhs ester

Atto 590 nhs ester | 670269-33-7

Catelog Number F10-0097
Category ATTO
Molecular Formula C41H42ClN3O11
Molecular Weight 788.24
Catalog Number Size Price Quantity
F10-0097 -- $--

Product Introduction

ATTO 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The ATTO dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.ATTO 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. ATTO 590-N-hydroxysuccinimide (NHS) is membrane permeable.

Chemical Information

Synonyms 2-(6,20-diethyl-7,7,9,17,19,19-hexamethyl-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03,12.05,10.016,21; docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl)-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoic acid;perchlorate; 6-(2-Carboxy-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}phenyl)-1,11-diethyl-2,2,4,8,10,10-hexamethyl-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium perchlorate
Purity ≥60% (coupling to amines)
IUPAC Name 2-(6,20-diethyl-7,7,9,17,19,19-hexamethyl-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl)-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoic acid;perchlorate
Canonical SMILES CCN1C2=CC3=C(C=C2C(=CC1(C)C)C)C(=C4C=C5C(=CC([N+](=C5C=C4O3)CC)(C)C)C)C6=C(C=CC(=C6)C(=O)ON7C(=O)CCC7=O)C(=O)O.[O-]Cl(=O)(=O)=O
InChI InChI=1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)
InChI Key SHJDVERDESTYRQ-UHFFFAOYSA-N
MDL Number MFCD03456133
NACRES NA.32

Product Specification

Excitation 590
Emission ~620
Properties Quality Level 100
Storage −20 °C
Application Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data)ATTO 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.

Computed Properties

Hydrogen Bond Donor Count 1
Hydrogen Bond Acceptor Count 12
Rotatable Bond Count 7
Exact Mass 787.2507869 g/mol
Monoisotopic Mass 787.2507869 g/mol
Topological Polar Surface Area 191Ų
Heavy Atom Count 56
Formal Charge 0
Complexity 1770
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 2
Compound Is Canonicalized Yes

Patents

Publication NumberTitlePriority Date
CN-109135749-B Hybrid composite fluorescent powder and preparation method and application thereof 2018-09-29
CN-109923407-A Sugar chain analysis method 2016-11-07
EP-3537142-A1 Glycan analysis method 2016-11-07
JP-WO2018083790-A1 Sugar chain analysis method 2016-11-07
US-2019317101-A1 Glycan analysis method 2016-11-07
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