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Aminoallyl-dUTP-ATTO-647N
| Catalog Number | A07-0035 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C58H78N9O15P3(freeacid) |
| Molecular Weight | 1151.08 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A07-0035 | -- | $-- |
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Product Introduction
Aminoallyl-dUTP-ATTO647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-ATTO647N/dTTP ratio for Nick Translation: 30-50% Aminoallyl-dUTP-ATTO647N/ 50% dTTP
Chemical Information |
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|---|---|
| Purity | ≥95% (HPLC) |
| pH | 7.5 ±0.5 |
| Appearance | sterile blue solution in 10 mM Tris-HCl |
- Product Specification
- Application
| Spectroscopic Properties | λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Aminoallyl-dUTP-ATTO-647N is a fluorescently labeled nucleotide analog used in a variety of molecular biology applications. Here are some key applications of Aminoallyl-dUTP-ATTO-647N:
Fluorescent Labeling of Nucleic Acids: Aminoallyl-dUTP-ATTO-647N can be incorporated into DNA or RNA during PCR or in vitro transcription reactions. This incorporation allows for the direct visualization of nucleic acids under a fluorescence microscope or in fluorescence-based assays. The high sensitivity and specificity of ATTO-647N fluorophore make it ideal for detailed imaging and tracking of nucleic acids in complex biological samples.
DNA Microarray Analysis: In DNA microarray experiments, Aminoallyl-dUTP-ATTO-647N is used to label cDNA or cRNA, allowing for the detection and quantification of gene expression levels. The bright and stable fluorescence of ATTO-647N ensures accurate and reliable signal detection across the microarray. This application is crucial for gene expression profiling, biomarker discovery, and comparative genomics studies.
FISH (Fluorescence In Situ Hybridization): Aminoallyl-dUTP-ATTO-647N is employed in FISH protocols to label DNA probes that hybridize to specific chromosomal regions. This enables the visualization of chromosomal aberrations, gene localization, and genome organization in cells and tissues. The superior photostability of ATTO-647N helps maintain fluorescence signal integrity during prolonged imaging sessions, enhancing the accuracy of FISH analyses.
Quantitative PCR (qPCR): Incorporation of Aminoallyl-dUTP-ATTO-647N into qPCR enables real-time monitoring of DNA amplification with high sensitivity. The fluorescence emitted by ATTO-647N can be quantitatively measured during each amplification cycle, providing precise quantification of target DNA sequences. This application is essential for various genetic and diagnostic studies, including pathogen detection, SNP genotyping, and quantification of gene expression.
Applications of Fluorescent Probes & Dyes
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