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Basic Yellow 2 | 2465-27-2
| Catalog Number | A01-0010 |
| Category | Fluorescent Proteins, Nucleotides & Other Conjugates |
| Molecular Formula | C17H21N3.HCl |
| Molecular Weight | 303.83 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A01-0010 | -- | $-- |
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Product Introduction
Basic Yellow 2, a substituted diphenyl-methane cationic dye, is used as a probe for studies of microheterogeneous systems, from micelles, proteins, and polymers to silica surfaces.
Chemical Information |
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|---|---|
| Related CAS | 492-80-8 (free base) 12237-78-4 (Deleted CAS) |
| Synonyms | C.I. Basic Yellow 2; C.I. Basic Yellow 2, monohydrochloride; ADC Auramine O; Aizen Auramine OH; Arazine Yellow; Auramin; Auramine; Auramine chloride; Basic yellow O; Calcozine Yellow OX; Flexo Yellow 110; Mitsui Auramine O; Auramine O |
| Purity | >80.0%(LC)(T) |
| IUPAC Name | 4-[4-(dimethylamino)benzenecarboximidoyl]-N,N-dimethylaniline;hydrochloride |
| Canonical SMILES | CN(C)C1=CC=C(C=C1)C(=N)C2=CC=C(C=C2)N(C)C.Cl |
| InChI | InChI=1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H |
| InChI Key | KSCQDDRPFHTIRL-UHFFFAOYSA-N |
| Solubility | Soluble in DMSO (Slightly), Methanol (Slightly) |
| Appearance | Yellow to Dark Orange Solid |
| Melting Point | >250°C (dec.) |
| MDL Number | MFCD00012484 |
- Product Specification
- Application
| Maximum Absorption Wavelength | 432(H2O) nm |
| Storage | Keep container tightly closed. Store in a cool and dark place. |
Basic Yellow 2, also known as auramine O, is a versatile fluorescent dye with a multitude of applications across various fields. Here are four key applications of Basic Yellow 2:
Microscopy: In the realm of fluorescence microscopy, Basic Yellow 2 emerges as a pivotal tool for staining acid-fast bacteria, particularly those from the Mycobacterium genus. By intricately binding to the cell walls of these bacterial entities, it enables crystal-clear visualization under the discerning eye of a fluorescence microscope. This technique stands as a linchpin in the diagnosis of tuberculosis and various mycobacterial infections, allowing for the precise identification of these elusive pathogens.
Histology: Diving into the intricate world of histological studies, Basic Yellow 2 plays a critical role in accentuating tissues and cells, enhancing the delineation of specific structures as they unfold under the meticulous gaze of a microscope. By amplifying contrast and aiding in the demarcation of cellular components, this dye empowers pathologists in scrutinizing tissue samples, assisting in the diagnosis of a spectrum of medical conditions and revealing hidden complexities within biological specimens.
Flow Cytometry: Rising as a prized asset in the domain of flow cytometry, Basic Yellow 2 transforms into a radiant fluorescent marker, crucial for the sorting and analysis of cells. By harnessing the vibrant hues of this dye to illuminate cells, researchers can distinguish distinct populations based on their unique fluorescence signatures, unlocking a realm of immunophenotyping possibilities.
Applications of Fluorescent Probes & Dyes
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