3,3'-Dipropylthiadicarbocyanine Iodide | 53213-94-8

3,3’-Dipropylthiadicarbocyanine Iodide, known as DiSC3(5), is a cyanine dye commonly utilized in research settings for membrane potential and cellular dynamics. Here are four key applications of DiSC3(5):

Membrane Potential Studies: DiSC3(5) stands as a cornerstone tool for probing membrane potential fluctuations in diverse cell types, encompassing neurons and cardiac cells. By integrating into cell membranes, it dynamically responds to voltage variations through fluorescence alterations, furnishing real-time insights into cellular excitability. This sophisticated capability finds particular significance in electrophysiological investigations, shedding light on the intricate realm of ion channel kinetics and dynamics.

Bacterial Viability Assays: DiSC3(5) emerges as a pivotal agent in evaluating bacterial viability and susceptibility to antibiotics. Entrapped within intact bacterial cell membranes, the dye emits fluorescence which diminishes upon membrane rupture, facilitating swift discrimination between live and deceased bacterial populations. This swift and precise discrimination plays a pivotal role in microbiological inquiries and drug screening endeavors, accelerating progress in these domains.

Mitochondrial Function Analysis: Delving into the heart of mitochondrial health assessment, DiSC3(5) serves as a potent tool for tracking changes in mitochondrial membrane potential, providing invaluable insights into these vital organelles. Functioning mitochondria uphold an electrical gradient across their membranes, a phenomenon vividly highlighted by this dye. This analytical prowess is instrumental in scrutinizing cellular energetic processes, apoptotic pathways, and a spectrum of mitochondrial disorders, unlocking deeper understandings of cellular dynamics.

Flow Cytometry: Within the realm of flow cytometry, DiSC3(5) assumes a pivotal role as a fluorescent tag for characterizing cell populations based on their membrane potential attributes. This technology enables researchers to discern distinct cellular states or types within heterogeneous samples, fostering nuanced analyses of cellular behavior. The amalgamation of DiSC3(5) with other fluorescent markers enables comprehensive multiparametric assessments of cells, catering to the intricate needs of both research endeavors and clinical diagnostics.