3,3'-dioctadecylthiacarbocyanine perchlorate | 53533-50-9

3,3’-Dioctadecylthiacarbocyanine perchlorate, commonly known as DiOC18(3), is a versatile fluorescent probe with a broad range of applications in cell biology. One of its key applications is in the study of mitochondrial membrane potential. DiOC18(3) is a cationic dye that accumulates in the negatively charged mitochondrial matrix in healthy cells, thereby allowing researchers to assess the functional status of mitochondria. Loss of mitochondrial membrane potential is a hallmark of early apoptosis; hence, this dye is indispensable for experiments related to cell health, apoptosis, and the efficacy of drugs targeting mitochondrial function. It provides valuable insights into cell metabolism and bioenergetics, highlighting the dye’s critical role in understanding mitochondrial dynamics.

Another significant application of 3,3’-dioctadecylthiacarbocyanine perchlorate is in the study of lipid bilayers and membrane dynamics. DiOC18(3)'s lipophilic properties enable it to integrate seamlessly into cell membranes, making it useful for visualizing cell membrane integrity, fluidity, and organization. This application is particularly useful in neurobiology, where membrane dynamics are crucial for synaptic function and neuron signaling. Moreover, DiOC18(3) aids in assessing the interactions between various lipids and proteins within the membrane, providing detailed snapshots of cell membrane architecture, which are pivotal for advancements in drug delivery systems and membrane biophysics.

Furthermore, DiOC18(3) serves an important role in multi-parametric flow cytometry analyses. This dye can be employed to label live cells, enabling the detection of changes in cellular physiology under different experimental conditions. Its compatibility with flow cytometry allows for high-throughput analysis of large cell populations, facilitating the study of cell cycle progression, viability, and population dynamics. By using DiOC18(3) in conjunction with other fluorescent markers, researchers can obtain a comprehensive understanding of cell behavior and characteristics in various environments, making it an invaluable tool for both basic and applied cell research.

Lastly, 3,3’-dioctadecylthiacarbocyanine perchlorate is instrumental in tracking cell migration and proliferation. Due to its stable fluorescence, DiOC18(3) can be used in long-term live cell imaging studies to monitor the movement and division of cells over time. This is particularly beneficial for cancer research, where understanding the invasive and metastatic behavior of tumor cells is crucial. The dye’s ability to consistently label cell membranes without significantly affecting cell viability makes it an excellent choice for real-time studies of cellular dynamics. The insights gained from these studies can lead to the development of targeted therapies that can effectively inhibit cancer progression and metastasis.